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KMID : 0616620020080010023
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Journal of Soonchunhyang Medical College
2002 Volume.8 No. 1 p.23 ~ p.29
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Expression of p53 and p21 in actinic keratoses, keratoacanthoma and Bowen
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Abstract
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Background: The function of the p53 protein was known to regulate cell proliferation by inhibiting cells in S phase, so DNA damaged cell proliferation was not occured by apoptosis. p21 is a cyclin dependent kinase inhibitor induced by wild type p53. p21 is thought to mediate the signal of p53 induced by DNA damaged agents and to arrest the cell cycle in G1 phase. p21 and p53 were expressed in many malignant tumors, and its role in oncogenesis, tumor progression and prognosis were important. The authors analyzed immunohistochemical expression of mutant p53 and p21 protein in keratocarcinoma, squamous carcinoma and basal cell carcinoma.
Method: Eight cases of keratocarcinom, fifteen cases of squamous cell carcinoma and twenty cases of basal cell carcinoma were immunohistochemically stained with p21 and p53 monoclonal antibodies.
Results:
1. The positive reaction of p21 protein and p53 protein in keratocarcinoma were samely observed in 7/8 cases(87%) and the positive reaction of p21 and p53 protein in squamous cell carcinoma were samely observed in all cases (100%)
2. The reaction pattern of p21 protein and p53 protein showed that positive reaction in both basal layer and keratinocytes concomitantly were more common observed that that in basal layer only. But the case of positive reaction in only keratinocytes was not observed.
3. In basal cell carcinoma, the positive reaction of p21 protein were seen in 5/20 cases(25%) and that of p53 were positive reaction in 15/20 cases(75%). p21 protein also showed positive reaction in cases of p53 protein positive cases.
4. The reaction pattern in basal cell carcinoma showed more intense reaction in peripheral portion than central portion of tumor nests, which suggested that tumor cells in the peripheral portion was composed of less mature cells than that of cental portion. The mutant p53 protein had lost of capability of inducing p21 protein. So in cases of p53 protein was negative, the p21 protein theoretically showed negative reaction. But the p21 protein positive cases were observed in p53 positive cases, which was suggested that the p21 protein was induced by the p53 independent pathway. The prognosis appeared to be good in cases of both p21 protein(+) and p53 protein(+) cases than that of p21(-) and p53(+).
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